Mycobacterium Tuberculosis Strains Infection Caused Different Level of Apoptosis Macrophage Cells Derived from Pulmonary Tuberculosis Patients in Indonesia

Yanti, Budi and Mulyadi, . and Soetjipto, . and Mertaniasih, Ni Made and Amin, Muhammad (2019) Mycobacterium Tuberculosis Strains Infection Caused Different Level of Apoptosis Macrophage Cells Derived from Pulmonary Tuberculosis Patients in Indonesia. Bali Medical Journal, 8 (3). p. 22. ISSN 2089-1180

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Official URL: https://www.balimedicaljournal.org/index.php/bmj/a...

Abstract

Introduction: Mycobacterium tuberculosis (MTB) is the causative agent of human tuberculosis and facultative intracellular pathogen of macrophage cells. Apoptosis macrophage plays a critical role for the host defence to resist MTB infection. The Beijing strain of MTB is regarded as “hypervirulent” due to its ability to inhibit apoptosis macrophage, expand the infection and disease of tuberculosis. Methods: Active pulmonary tuberculosis patients were diagnosed based on AFB sputum smear, gene Xpert MTB/RIF, and chest X-ray. Bronchoalveolarlavage was performed to specify specimens and identify MTB strain using RD9 and TbD1 primers by PCR methods, respectively. Detection of MTB strain by genome sequencing and DNA homology analysis can lie performed with BLAST programs. Apoptosis level was measured by TUNEL assay methods. Results: MTB strain were detected in all patients in which 24 and 6 isolates and classified as the hypervirulent homologous of Beijing strains and 6 isolates of the Mycobacterium bovis BCG homologous strain, respectively. Apoptosis level detected decrease in macrophage cells derived from patients with hypervirulent homologous of Beijing strains infection compared with Mycobacterium bovis strain infection (p <0.05) Conclusion: A homologous Beijing strain and Mycobacterium bovis strain were detected among pulmonary TB patients in Indonesia. A hypervirulent strain infections have a great ability to inhibit apoptosis of macrophage cells in human. Therefore, laboratory test to determine MTB strains should be integrated in National Tuberculosis Control Program in the country.

Item Type: Article
Uncontrolled Keywords: pulmonary tuberculosis, MTB strain, bronchoalveolar lavage, apoptosis macrophage
Subjects: R Medicine > RC Internal medicine > RC306-320.5 Tuberculosis
Divisions: Faculty of Medicine > Program Study of Medicine
Depositing User: Mr. . Aji
Date Deposited: 23 Jun 2020 04:37
Last Modified: 23 Jun 2020 05:02
URI: http://repository.unusa.ac.id/id/eprint/6138

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