Handayani, . and HANDAJANI, RETNO and Susilo, Imam and Basori, Achmad and Salim, Hotimah Masdan (2020) Apoptotic Effect Of Physalis Minima Linn Ethanol Extract On Breast Cancer Cells Via P53 Wild-Type And Apaf-1 Protein. Tropical Journal of Pharmaceutical Research, 19 (10). pp. 2085-2089. ISSN 1596-9827
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Abstract
Purpose: To produce an anti-cancer agent from Physalis minima ethanol extract as well as prevent the growth of NMU-induced breast cancer and MCF-7 cell line. Methods: This research used an animal model (Wistar-Furth rats), and cell line used in this study was normal breast-cell line MCF-7. The rats were administered with the ethanol extract of Physalis minima Linn (100, 250 and 400 mg/kg/day) by gavage, once a day for 4 weeks. Meanwhile, MCF-7 cell lines were cultured in medium and incubated in 100 μg/mL of ethanol extract of Physalis minima for 48 h. The samples were analyzed using histology and immunohistochemistry techniques for expression of p53 antibody DO-1 and APAF-1. Results: The results of immunohistological analysis in the breast organ showed that Physalis minima Linn extract significantly (p < 0.05) increased the tumor suppressor protein p53 at doses of 100, 250 and 400 mg/kg/day. In addition, the extract also significantly (p < 0.05) increased APAF-1, which is a gene determining cell death, at doses of 100, 200 and 400 mg/day. Conclusion: Ethanol extract of Physalis minima Linn inhibits the cytotoxic activity of NMU-induced breast cancer by increasing the tumor suppressor protein p53 and APAF-1. Thus, Physalis minima Linn extract can potentially be used as a complementary treatment for inhibiting the growth of breast cancer cells in patients.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Apoptosis protease-activating factor-1, Breast cancer, MCF-7 cell line, Physalis minima, p53 |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine > Program Study of Medicine |
| Depositing User: | Mr. . Aji |
| Date Deposited: | 27 Aug 2021 03:52 |
| Last Modified: | 12 Dec 2022 04:48 |
| URI: | http://repository.unusa.ac.id/id/eprint/6634 |
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