Arifin, S and Handono, K and Poeranto, Sri and Nurdiana, N and Hartanti, KD and Albaar, TM and Gumilang, Retna and Dharmesta, NA (2019) Desensitization with Self-Antigen dsDNA Inhibits B and T-cell Functions by Modulating Treg as Regulator Immune System in Pristane-induced Lupus Mice Model. Journal of Stem Cell Research and Tissue Engineering, 3 (1). pp. 12-21. ISSN 2614-1256
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Abstract
The aim of this study was to develop a novel therapeutic method for improving immune system regulation in SLE using escalating dose self-antigen dsDNA immunotherapy. Methods: Female Balb/c mice were given a single intraperitoneal injection of 0.5 ml pristane.. Starting at 12 weeks after injection,. the mice were evaluated for clinical and serological manifestations. Mice with lupus signs (PIL mice) were divided into four groups; positive control group, PIL A (0.01 μg/ml, 0.1 μg/ml, 1 μg/ml ) EDI dsDNA, PIL B (0.1 μg/ml, 1 μg/ml, 10 μg/ml ) EDI dsDNA, and PIL C(1 μg/ml, 10 μg/ml, 100 μg/ml). EDI dsDNA were administered once every week in consecutively. The doses would increase every week. dsDNA were complexed with the cationic polyethylenimine (PEI) before injection. Samples were analyzed for autoantibodies levels (dsDNA, ANA) and ,TGF-β cytokine from serum using ELISA and T-Reg, mature dendritic cells from spleen using flowcytometry.. Results: Escalating dose antigen spesific immunotherapy with self-antigen dsDNA significantly decreased ANA (p=0.02), anti-dsDNA (p=0.03), dendritic cell mature (p=0.02) compare to positive control, and not significantly decreases Th17 cells (p=0,18) but the result tend to get lower. Desensitization using self-antigen dsDNA was increased T-reg proliferation (p=0.00) and level of TGF-β (p=0.03) significantly compare to positive control. Conclusion: Desensitization using self-antigen dsDNA coupled to PEI was able to modulate T-Reg as a regulator immune respon and inhibit B and T cell functions in lupus mice model.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Systemic Lupus Erythematosus, dsDNA, escalating dose, tolerance, Treg |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine > Program Study of Medicine |
| Depositing User: | Mr. . Aji |
| Date Deposited: | 07 Jun 2022 03:44 |
| Last Modified: | 07 Jun 2022 03:44 |
| URI: | http://repository.unusa.ac.id/id/eprint/8623 |
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