Putri, Winawati Eka and Endaryanto, Anang and Tinduh, Damayanti and Alinda, Medhi Denisa and Prakoeswa, Cita Rosita Sigit (2021) Growth factors of hypoxia Freeze-Dried Adipose Stem Cell- Conditioned Medium (FD ASC-CM) and Fresh Adipose Stem Cell Conditioned-Medium (FR ASC-CM): a comparative study. Bali Medical Journal, 10 (2). pp. 683-687. ISSN 2302-2914
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Growth factors of hypoxia Freeze-Dried Adipose Stem Cell- Conditioned Medium (FD ASC-CM) and Fresh Adipose Stem Cell Conditioned-Medium (FR ASC-CM) a comparative study.pdf Download (513kB) | Preview |
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Abstract
Background: Adipose Stem Cells (ASC) are multipotent stem cells which mechanism of action is mainly secretion of various paracrine molecules known as secretome or conditioned medium (CM). Adipose Stem Cell-Conditioned Medium (ASC-CM) collects several soluble factors, such as cytokines, chemokines, and Growth Factors (GF) secreted by almost all types of living cells in the extracellular space. The freeze-dried (FD) form of ASC-CM can reduce the instability of the fresh (FR) form of ASC-CM. This study aims to compare the GF levels between FD ASC-CM and FR ASC-CM as a comparative study. Methods: ASC-CM was prepared from human adipose tissue. After we got the FR ASC-CM, some of them were changed into an FD form. We measured the several GF levels using ELISA methods in ng/L. Data were presented in mean and standard deviation as well as analyzed using SPSS version 25 for Windows. Results: The average levels of EGF in both FD ASC-CM and FR ASC-CM were 256.80±9.70 ng/L and 290.20±53.20 ng/L, followed by TGF-? (564.20±12.70 ng/L and 633.10±11.40 ng/L), PDGF (2.90±0.19 ng/L and 2.90±0.20 ng/L), and VEGF (711.10±21.70 ng/L and 788.70±204.80 ng/L) respectively. There was a statistically significant difference in TGF-? levels between FD ASC-CM and FR ASC-CM (p=0.002). However, there was no a significant difference in EGF, PDGF, and VEGF levels between FD ASC-CM and FR ASC-CM (p>0.05) Conclusion: There was a statistically significant difference between FD and FR ASC-CM only in TGF-? levels.
| Item Type: | Article |
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| Uncontrolled Keywords: | ASC-CM, Growth Factors, Freeze-Dried, Fresh |
| Subjects: | R Medicine > RV Botanic, Thomsonian, and eclectic medicine |
| Divisions: | Faculty of Medicine > Program Study of Medicine |
| Depositing User: | Mr. . Aji |
| Date Deposited: | 31 Jan 2023 07:36 |
| Last Modified: | 31 Jan 2023 07:36 |
| URI: | http://repository.unusa.ac.id/id/eprint/9169 |
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