Krisnawati, Dyah Ika and Hsu, Po-Hsuan and Lin, Yu-Hsiang and Alimansur, Moh and Atmojo, Didik Susetiyanto and Rahmawati, Elfi Quyumi and Rahayu, Dwi and Khafid, Muhammad and Lu, Ssu-Chiao and Kuo, Tsung-Rong (2021) The Use of the ROS Scavenger Cysteine as a Surface Ligand of Metal Nanoclusters and Its Bactericidal Elimination Effect. Applied Sciences, 11 (9). pp. 1-12. ISSN 2076-3417
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Abstract
The bactericidal effects of fluorescent metal nanoclusters have impeded their bacterial bioimaging applications due to the reactive oxygen species (ROS) generation that is induced by the nanoclusters in bacteria to cause bacterial death. Herein, an ROS scavenger of cysteine was exploited as a surface ligand to prepare cysteine-conjugated gold nanoclusters (Cys–AuNCs) and cysteine-conjugated silver nanoclusters (Cys–AgNCs) using a facile hydrothermal approach. The structural and optical characterizations demonstrated successful syntheses of Cys–AuNCs and Cys–AgNCs. With the same weight concentration, the bactericidal effect increased in the order of Cys–AuNCs, Cys–AgNCs, and silver nanoparticles (AgNPs), according to the results of the bacterial growth curves. Furthermore, based on the results of the standard colony-counting method, the Cys–AuNCs revealed the best biocompatibility compared to those of the Cys–AgNCs and AgNPs in Escherichia coli (E. coli). The superior biocompatibility of the Cys–AuNCs can be attributed to the use of the ligand of cysteine as an ROS scavenger to reduce ROS in E. coli. Electron paramagnetic resonance (EPR) analyses indicated that the use of the ROS scavenger cysteine as the surface ligand of the Cys–AuNCs eliminated the ROS production induced by the Cys–AuNCs in E. coli. The biocompatible Cys–AuNCs were also confirmed as a fluorescent probe using confocal microscopy. Highly biocompatible Cys–AuNCs could be a potential fluorescent probe in the application of bacterial bioimaging.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | cysteine; reactive oxygen species; scavenger; nanoclusters; bactericidal effect |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Faculty of Nursing and Midwifery > Program Study of Nursing Bachelor |
| Depositing User: | Mr. . Aji |
| Date Deposited: | 24 Oct 2022 03:07 |
| Last Modified: | 24 Oct 2022 03:07 |
| URI: | http://repository.unusa.ac.id/id/eprint/8958 |
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